Coding

Part:BBa_K4382006:Design

Designed by: Ashwin Ananthanarayanan   Group: iGEM22_IISER_Bhopal   (2022-10-10)


Endo Pectin Lyase - PelB-B2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 731


Design Notes

The gene is codon optimized for cloning into Bacillus subtilis 168.



Source

PelB-B2 is originally present in Bacillus sp.(OR-B2).

References